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T4 DNA Ligase

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.

T4 Polynucleotide Kinase

PNK catalyses the transfer of terminal phosphate from the gamma position of ATP to the 5`-hydroxyl terminus of polynucleotides and nucleoside 3`-monophosphates.