Aminoallyl-dUTP, 10 mM

Aminoallyl-dUTP (AA-dUTP, 10 mM) is a modified nucleotide used for indirect non-radioactive DNA labeling.
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Description

AA-dUTP ( 5- (3-aminoallyl)- 2′- deoxyuridine -5’- triphosphate, trilithium salt) is used for indirect DNA labeling. The highly purified nucleotide can be enzymatically incorporated into DNA with Taq DNA Polymerase, Klenow Fragment or Reverse Transcriptase. DNA labeling is possible with biotin, hapten or any amine-reactive fluorescent dye. AA-dUTP is supplied as aqueous solution with a concentration of 10 mM.

Storage at -20 °C.

Applications

  • Real-Time PCR
  • RT-PCR
  • Random priming
  • Nick-translation

Datasheets

IFU_513001_Amino-dUTP_Rev01_0819_rj