RealLine HBV qualitative

The RealLine HBV qualitative kit detects the different genotypes of HBV DNA by Real-time PCR. The kits provide qualitative detection.

RUO – Research Use Only

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Description

Hepatitis B Virus HBV is a partially double-stranded DNA virus of the Hepadnaviridae family that causes acute and chronic liver disease with a very high risk of death from liver cirrhosis and cancer. Worldwide an estimated 2 billion people have been infected with the hepatitis B virus (HBV) and more than 350 million have chronic consequences of hepatitis B.

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for HBV DNA

Formats

Str-format:

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

IF you have questions to the use of your cycler, please contact us!

Data sheets