RealLine HBV quantitative incl. Extraction

The RealLine HBV quantitative kit detects the different genotypes of HBV DNA by Real-time PCR. The kits provide quantitative detection.

The components for the extraction of the DNA are included.

RUO – Research Use Only

Clear
Categories: , SKU: N/A

Description

Hepatitis B Virus HBV is a partially double-stranded DNA virus of the Hepadnaviridae family that causes acute and chronic liver disease with a very high risk of death from liver cirrhosis and cancer. Worldwide an estimated 2 billion people have been infected with the hepatitis B virus (HBV) and more than 350 million have chronic consequences of hepatitis B.

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for HBV DNA

Formats

Uni-format:

The Ready-Mastermix is lyophilized  and prepared in 0.2 ml clear single tubes.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes their caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, Rotor-Gene Cyclers. BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Data sheets

IFU_HBV_VBD0599_Rev06_1119_EN