RealLine HCV qualitative, incl. Extraction

The RealLine HCV qualitative Kit is intended for a sensitive detection of Hepatitis-C Virus.

The components for the extraction of the RNA are included.

RUO – Research Use Only

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Description

Hepatitis C virus (HCV) is a public health concern worldwide and a major cause of chronic liver inflammation, cirrhosis and hepatocellular carcinoma. The Hepatitis C virus is transmitted parenterally, showing a high incidence in hemophiliacs hemodialysis patients, injecting drug users and patients with post-transfusion hepatitis.

Detection channels

  • FAM (495 – 520 nm) for Internal Control
  • ROX (575 – 602 nm) for HCV cDNA

Formats

Uni-format

The Ready-Mastermix is lyophilized  and prepared in 0.2 ml clear single tubes.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes their caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, Rotor-Gene Cyclers. BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

If you have questions to the use of your cycler, please contact us!

Data sheets