RealLine HCV qualitative

The RealLine HCV qualitative Kit is intended for a sensitive detection of Hepatitis-C Virus.

RUO – Research Use Only

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Description

Hepatitis C Virus (HCV) is a public health concern worldwide and a major cause of chronic liver inflammation, cirrhosis and hepatocellular carcinoma. The Hepatitis C virus is transmitted parenterally, showing a high incidence in hemophiliacs hemodialysis patients, injecting drug users and patients with post-transfusion hepatitis.

Detection channels

  • FAM (495 – 520 nm) for Internal Control
  • ROX (575 – 602 nm) for HCV cDNA

Formats

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

If you have questions to the use of your cycler, please contact us!

Data sheets