RealLine CMV quantitative

The RealLine CMV quantitative kits are designed for sensitive detection of CMV DNA in clinical samples by Real-Time PCR. The kit is designed for in vitro Diagnostics and can be used for a quantitative determination of the viral load.

All components are lyophilized and ready-to use.

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Description

The Cytomegalo Virus CMV is commonly carried by the general population (50 to 80 %). CMV infection is important for certain high-risk groups: patients who are immunocompromised e.g. patients with HIV, organ transplant recipients, persons with leukmia, pre-natal and postnatal infants. Therewith CMV is one of the TORCH infections that lead to congenital abnormalities and therefore an early diagnosis of CMV especially for pregnant women and women in the reproductive age is extremely important.

Sample Material

  • Scrapings of epithelial cells
  • Saliva
  • Semen
  • Prostatic fluid
  • Urine
  • Whole blood, serum, plasma, leukocytic mass

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Cytomegalovirus CMV DNA

Formats

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

Data sheets

IFU_CMV_VBD1596_Rev09_0919_EN

IFU_CMV_VBD1598_Rev09_0919_EN