RealLine HIV qualitative incl. Extraction

The RealLine HIV qualitative Kit is intended for a sensitive detection of RNA of the Human Immunodeficiency Virus (HIV) by Reverse Transcription and Real-Time PCR in one tube.

The components for the extraction of the RNA are included.

RUO – Research Use Only



The Human immunodeficiency Virus (HIV) is a member of the genus Lentivirus, part of the family of Retroviridae. This virus causes the acquired immunodeficiency syndrome (AIDS). From its discovery in 1981 AIDS killed more than 25 million people and is worlwide spread.

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for HIV cDNA



The Ready-Mastermix is lyophilized  and prepared in 0.2 ml clear single tubes.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes their caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, Rotor-Gene Cyclers. BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Data sheets