RealLine SARS-CoV-2 lyo

The RealLine SARS-CoV-2 lyo kit is intended for the detection of RNA of RdRP-Gene and N-Gene from SARS-CoV-2 by Real-Time-PCR method.

All components are lyophilized and ready-to use.



The RealLine SARS-CoV-2 lyo kit detects the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, formerly 2019-nCoV). This novel virus belongs to the corona virus family and from January 2020, it was discovered that this virus is responsible for infections that spread rapidly around the world. A few days after the first report of patients with pneumonia of unclear origin, SARS-CoV-2 was identified as the causative agent. SARS-CoV-2 is mainly transmitted by droplet infection when coughing or sneezing and in close contact with infected persons.

Sample Material

  • Nasopharyngeal and oropharyngeal swabs
  • Sputum,
  • Bronchoalveolar lavage
  • Fluid

Detection Channels

  • FAM for Internal Control (RNA-IC)
  • ROX for RdRP- and N-genetargets of the SARS-CoV-2 Virus RNA



In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

Data sheets