RealLine Candida albicans / Gardnerella vaginalis

The RealLine Candida albicans / Gardnerella vaginalis kit is detecting and differentiating DNA from Candida albicans and Gardnerella vaginalis by Real-Time PCR.

All components are lyophilized and ready-to use.

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Description

Candida albicans can cause Candidiasis. Such infections range from superficial, such as oral thrush and vaginitis, to systemic and potentially life-threatening diseases.  Candida infections of the latter category are also referred to as candidemia and are usually confined to severely immunocompromised persons, such as cancer, transplant, and AIDS patients, as well as nontrauma emergency surgery patients.

Gardnerella vaginalis is a facultatively anaerobic Gram-variable rod that can cause bacterial vaginosis in some women as a result of a disruption in the normal vaginal microflora.

Sample Material

  • Saliva
  • Urine
  • Scrapings of epithelial cells

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Candida albicans DNA
  • ROX (575 – 602 nm) for Gardnerella vaginalis DNA

Formats

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

Data sheets

IFU_CA_GV_VBD0444_Rev04_0919_EN

IFU_CA_GV_VBD0445_Rev04_0919_EN