DF-Pfu Polymerase

DF-Pfu Polymerase is a thermostable enzyme with proofreading activity and suitable for all PCR amplifications with require high-fidelity.

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Description

DF-Pfu DNA polymerase 5 U/µl, is a thermostable Polymerase with 3′ to 5′ exonuclease proofreading activity. It was isolated from hyperthermophilic Pyrococcus furiosus with a size of 92 kDa. DF-Pfu Polymerase has a 12-fold increased fidelity compared to Taq DNA polymerases and is therefore recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. DF-Pfu DNA polymerase generated PCR fragments are blunt-ended, which can be used directly for blunt end ligation.

Supplied with two different buffers (with and without MgSO4)  and additional MgSO4

Applications

* PCR and primer extension with high-fidelity
* blunt end ligation
* Amplification of bacterial sequences

Data sheets

IFU_108105_DF-Pfu_Rev01_0819_rj