BcI I

BcI I is a restriction enzyme purified from Bacillus caldolyticus.

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Description

BcI I is a restriction enzyme purified from Bacillus caldolyticus with a concentration of 10 U/µl. Restriction endonucleases are used in biotechnology for gene cloning, to cut DNA in smaller strands to study similarities and differences in fragment length among individuals like in RFLP (Restriction Fragment Length Polymorphism). BIORONs restriction enzymes show high purity and activity.

BcI I can not be heat-inactivated and shows 100% activity in BIORON buffers M and K (supplied with the enzyme).

5’…T▼GATCA…3’
3’…ACTAG▲T…5’

Application

  • Gene cloning
  • Protein production
  • RFLP (Restriction Fragment Length Polymorphism)

Datasheets

IFU_250104S_BclI_Rev01_0919_rj