BgI I

Bgl I is a restriction enzyme purified from Bacillus globigii lacking Bgl II.

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Description

Bgl I is a restriction enzyme purified from Bacillus globigii lacking Bgl II with a concentration of 10 U/µl. Restriction endonucleases are used in biotechnology for gene cloning, to cut DNA in smaller strands to study similarities and differences in fragment length among individuals like in RFLP (Restriction Fragment Length Polymorphism). BIORONs restriction enzymes show high purity and activity.

BgI I can be heat-inactivated after 20 min at 65°C and shows 100% activity in BIORON buffers M, H, SH and K (supplied with the enzyme). Please note that activity of BgI I is blocked by overlapping CpG methylation.

5’…GCCNNNN▼NGGC…3’
3’…CGGN▲NNNNCCG…5’

Application

  • Gene cloning
  • Protein production
  • RFLP (Restriction Fragment Length Polymorphism)

Datasheets

IFU_250105S_BglI_Rev01_0919_rj