BshF I (Hae III)

BshF I is a restriction enzyme purified from Bacillus sphaericus and is a isoschizomer to Hae III.

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Description

BshF I is a restriction enzyme purified from Bacillus sphaericus with a concentration of 10 U/µl. It is an isoschizomer of Hae III. Restriction endonucleases are used in biotechnology for gene cloning, to cut DNA in smaller strands to study similarities and differences in fragment length among individuals like in RFLP (Restriction Fragment Length Polymorphism). BIORONs restriction enzymes show high purity and activity.

BshF I can be heat-inactivated with 20 min at 80 °C and shows 100% activity in BIORON buffers A, M and K (supplied with the enzyme).

5’…AT▼CGAT…3’
3’…TAGC▲TA…5’

Application

  • Gene cloning
  • Protein production
  • RFLP (Restriction Fragment Length Polymorphism)

Datasheets

IFU_250110S_BshFI_Rev01_0919_rj